Human Butyrophilin Subfamily 1, Member A1 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com, designed for the in vitro quantitative determination of human BTN1A1 (Butyrophilin Subfamily 1, Member A1) in tissue homogenates or other biological fluids. The assay is supplied as a pre-coated 96-well microplate arranged in 12 x 8 well strips, with a total run time of approximately 4h 30m and a colourimetric readout read at 450nm in a microplate reader. The product is intended for research use only.

An antibody specific for BTN1A1 is pre-coated onto the plate; standards and test samples are added and any BTN1A1 present is captured. After washing, a Biotinylated Anti-BTN1A1 Antibody is added, followed by HRP-Avidin conjugate. TMB substrate is then used to visualise the reaction, producing a blue product that turns yellow upon addition of acidic stop solution; the intensity of yellow is proportional to BTN1A1 concentration. Sensitivity is <0.057 ng/ml with an assay range of 0.156–10 ng/ml.

Kit components include the pre-coated microplate, lyophilized standard, Detection Solutions A and B, Wash Buffer (30X), Sample Dilution Buffer, TMB Substrate, Stop Solution, and adhesive plate sealers, stored at +4°C or -20°C as indicated. Reported average recovery was 91% in serum (range 80–102%), 91% in EDTA plasma (81–101%), and 84% in heparin plasma (80–89%), with linearity data provided for dilutions of 1:2, 1:4, 1:8 and 1:16 across the same sample types.