Human Breast And Kidney Expressed Chemokine ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative measurement of human CXCL14 (Breast and Kidney Expressed Chemokine) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) with an immobilised antibody specific for CXCL14, a biotinylated anti-CXCL14 detection antibody, an HRP-avidin conjugate, and TMB substrate. Colour development is read at 450 nm on a colorimetric microplate reader and concentration is calculated from the standard curve. Total assay time is 4h 30m.

Reported sensitivity is 0.065 ng/ml, with a stated lower detection limit of <0.065 ng/ml and a quantitative working range of 0.156–10 ng/ml. In spike-and-recovery testing (n=5), average recovery was 91% in serum (range 80%–102%), 90% in EDTA plasma (range 81%–100%), and 84% in heparin plasma (range 80%–89%). Dilution linearity (n=5) across 1:2, 1:4, 1:8, and 1:16 dilutions gave 87%–91%, 87%–107%, 74%–101%, and 92%–97% in serum; 90%–105%, 84%–101%, 90%–101%, and 79%–108% in EDTA plasma; and 84%–95%, 92%–105%, 82%–105%, and 89%–91% in heparin plasma. Kit contents comprise the pre-coated plate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, stored at +4°C or -20°C as indicated; the kit should not be used past its expiration date.