Human Bone Morphogenetic Protein 2 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human BMP2 in serum, plasma, tissue homogenates or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, and follows a standard sandwich immunoassay workflow: a BMP2-specific capture antibody is immobilised on the plate, standards and samples are added, followed by a biotinylated anti-BMP2 detection antibody, then an HRP-avidin conjugate, with TMB substrate and an acidic stop solution. Absorbance is read at 450 nm using a colorimetric microplate reader, and BMP2 concentration is calculated against a standard curve.

The reported assay range is 46.8–3000 pg/ml with a sensitivity of <16.12 pg/ml. Total assay time is 4 hours 30 minutes. Spike-and-recovery performance in serum was 80%–102% (average 91%, n=5), in EDTA plasma 81%–100% (average 90%, n=5), and in heparin plasma 80%–89% (average 84%, n=5). Dilution linearity was demonstrated at 1:2, 1:4, 1:8, and 1:16 across the same sample types.

Supplied components include the pre-coated 96-well microplate, lyophilised standard (2 vials), detection solutions A and B (120 µl each), 30X wash buffer (20 ml), sample dilution buffer (45 ml), TMB substrate (9 ml), stop solution (6 ml), and plate sealers (5 adhesive strips). Components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.