Human Beta-1,3-Glucuronyltransferase 1 ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of human B3GAT1 (Beta-1,3-Glucuronyltransferase 1) in tissue homogenates, cell lysates or other biological fluids. The assay employs an antibody specific for B3GAT1 pre-coated onto a 96-well microplate configured as 12 x 8 well strips, with colorimetric detection read at 450 nm. The stated assay range is 0.156-10 ng/ml and the sensitivity is 0.053 ng/ml, with a total assay time of 4 hours 30 minutes.

Kit components include the pre-coated microplate, lyophilized standard, Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers, stored at +4°C or -20°C as instructed. Reported average recovery values for human serum, EDTA plasma and heparin plasma are 91%, 91% and 84% respectively, with ranges of 80-102%, 81-101% and 80-89% across serial dilutions of 1:2, 1:4, 1:8 and 1:16.