Human Basic Salivary Proline Rich Protein 2 ELISA Kit

Manufactured for the in vitro quantitative determination of human PRB2 in tissue homogenates and other biological fluids, this sandwich ELISA kit from Antibodies.com uses a pre-coated microplate format. The assay shows a sensitivity of 0.054 ng/ml across a working range of 0.156–10 ng/ml, with results read colorimetrically at 450 nm on a standard microplate reader. Total assay time is approximately 4 hours 30 minutes. The procedure uses a biotinylated anti-PRB2 detection antibody with HRP-avidin amplification, visualised via a TMB substrate that produces a yellow product whose intensity is proportional to PRB2 concentration in each well.

Reported sample recovery ranges are 80–102% in serum (n=5), 81–100% in EDTA plasma (n=5), and 80–89% in heparin plasma (n=5). Kit components include lyophilised standard, detection solutions A and B, wash buffer concentrate, sample dilution buffer, TMB substrate, stop solution and plate sealers, stored at +4°C or −20°C as instructed. The product is for research use only and is not intended for diagnostic or therapeutic applications.