Human Basic Salivary Proline Rich Protein 1 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Basic Salivary Proline Rich Protein 1 (p107) in saliva or other biological fluids. The assay uses a pre-coated 96-well microplate in a 12 x 8 well strip format and follows a standard sandwich enzyme immunoassay protocol with a biotinylated anti-p107 detection antibody and an HRP-avidin conjugate. Signal is developed with TMB substrate and quantified by reading the absorbance at 450 nm.

The reported assay range is 0.156-10 ng/ml with a sensitivity of <0.054 ng/ml, and the full protocol takes approximately 4 hours 30 minutes. Kit components — including the pre-coated microplate, lyophilized standard, detection solutions, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers — are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.