Human Bactericidal/Permeability Increasing Protein ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is intended for the in vitro quantitative determination of human Bpi (Bactericidal/Permeability Increasing Protein) in serum, plasma, tissue homogenates or other biological fluids. The microplate is pre-coated with an antibody specific for Bpi, and detection is performed colorimetrically by measuring absorbance at 450 nm on a microplate reader.

The assay has a sensitivity of <5.4 pg/ml and a working range of 12.5-800 pg/ml, with a total run time of 4h 30m. Reported recovery averages 91% (range 80%-102%) in serum, 90% (81%-100%) in EDTA plasma, and 84% (80%-89%) in heparin plasma, with dilutional linearity reported across multiple sample dilutions. Components supplied include a pre-coated microplate, lyophilized standard, Detection Solution A, Detection Solution B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution, and plate sealers, with storage at +4°C or -20°C as instructed.