Human B4GALT1 ELISA Kit

A sandwich ELISA kit from Antibodies.com intended for the quantitative measurement of human B4GALT1 [C0, S2]. The assay is reported as suitable for serum [S1], with a broader specification also covering plasma, tissue homogenates, and other biological fluids [S2]. Detection is colorimetric on a pre-coated microplate (12 x 8 well strips), read at 450 nm using a microplate reader, with biotinylated anti-B4GALT1 detection antibody, HRP-avidin conjugate, and TMB substrate producing a yellow chromogen after stop solution addition [S2]. Total assay time is 4h 30m [S2].

Reported sensitivity differs between sources: one gives <0.06 ng/ml with an assay range of 1.56 - 100 ng/ml [S1], while another gives 0.57 ng/ml (also described as <0.57 ng/ml) with an assay range of 1.56-100 ng/ml [S2]. Spike-and-recovery ranges reported for undiluted samples are 80% - 102% for serum, 81% - 101% for EDTA plasma, and 80% - 89% for heparin plasma, with additional dilution linearity data at 1:2, 1:4, 1:8, and 1:16 dilutions across the same sample types [S2].

Kit components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date [S2].