Human Autophagy Related Protein 7 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is designed for the in vitro quantitative determination of human ATG7 in tissue homogenates, cell lysates, or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a colorimetric readout based on TMB substrate. Absorbance is read at 450nm in a microplate reader, and the total assay time is 4 hours 30 minutes.

Quoted performance figures from the manufacturer include a sensitivity of 0.107 ng/ml and an assay range of 0.312-20 ng/ml. Recovery was evaluated across serum, EDTA plasma, and heparin plasma: serum (n=5) showed an average of 91% across a range of 80%-102%, EDTA plasma averaged 90% (81%-100%), and heparin plasma averaged 84% (80%-89%). Linearity was assessed at dilution factors of 1:2, 1:4, 1:8 and 1:16 across the same sample types, with corresponding recovery ranges reported for each matrix.

Kit components include a pre-coated microplate, lyophilized standard, Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and adhesive plate sealers. Storage conditions are +4°C or -20°C as indicated for individual components. The product is intended for research use only and is not for diagnostic or therapeutic applications.