Human Autophagy Related Protein 16 Like Protein 1 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is designed for the in vitro quantitative determination of human Autophagy Related Protein 16 Like Protein 1 (ATG16L1) in tissue homogenates or other biological fluids. The assay employs a pre-coated 96-well microplate (12 x 8 well strips), with standards and samples added to wells where ATG16L1 is captured by the immobilised antibody. Following incubation and washing, a biotinylated anti-ATG16L1 antibody is added, followed by HRP-avidin conjugate. The enzymatic reaction is visualised with TMB substrate, producing a blue product that turns yellow upon addition of acidic stop solution. Absorbance is read at 450 nm in a colorimetric microplate reader and the ATG16L1 concentration is calculated from the standard curve.

Reported performance figures include an assay range of 0.156-10 ng/ml and a sensitivity of 0.063 ng/ml. Mean recovery values across sample matrices (n=5) are 91% for serum (range 80%-102%), 91% for EDTA plasma (range 81%-101%), and 84% for heparin plasma (range 80%-89%). Dilution linearity across 1:2 to 1:16 dilutions is reported as 87-91% to 92-97% for serum, 90-105% to 79-108% for EDTA plasma, and 84-95% to 89-91% for heparin plasma. Total assay time is 4 h 30 m. Kit components include lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, stored at +4°C or -20°C depending on the component. The kit is intended for use before the stated expiration date.