Human Atrial Natriuretic Peptide ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of human Natriuretic Peptides A in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The kit uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, and analyte is quantified by absorbance at 450 nm on a colorimetric microplate reader.

The kit offers a product range of 15.6-1000 pg/ml with a sensitivity of 5.7 pg/ml and a total assay time of 4h 30m. Standards and test samples are added to the wells, where Natriuretic Peptides A is captured by the immobilized antibody. After washing, a biotinylated anti-Natriuretic Peptides A antibody is added, followed by HRP-Avidin conjugate and TMB substrate. The enzymatic reaction yields a blue product that converts to yellow upon addition of stop solution; the intensity of the yellow colour is directly proportional to Natriuretic Peptides A concentration, which is calculated against the standard curve.

Spike-and-recovery testing reported by the manufacturer gave averages of 91% in serum (range 80%-102%), 90% in EDTA plasma (range 81%-100%), and 84% in heparin plasma (range 80%-89%). Kit components are shipped for storage at +4°C or -20°C as instructed in the protocol, and the kit should not be used past its expiration date.