Human ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 ELISA Kit

From Antibodies.com, this Sandwich ELISA kit is designed for the in vitro quantitative determination of human SERCA2 ATPase (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2) in tissue homogenates, cell lysates and other biological fluids. It is listed under the Assays and Kits product category and is reactive with human samples.

The assay employs a pre-coated 96-well microplate arranged as 12 × 8 well strips and uses a sandwich enzyme immunoassay technique. SERCA2 ATPase captured on the plate is bound by a biotinylated anti-SERCA2 ATPase antibody and detected with an HRP-avidin conjugate, visualised using TMB substrate solution that produces a yellow product. Absorbance is read at 450 nm on a colorimetric microplate reader. The quantitative range is 0.312–20 ng/ml, with a sensitivity of <0.126 ng/ml, and the total assay time is 4 h 30 min.

Reported recovery averages were 91% in serum (n=5, range 80–102%), 91% in EDTA plasma (n=5, range 81–101%) and 84% in heparin plasma (n=5, range 80–89%). Dilution linearity across 1:2, 1:4, 1:8 and 1:16 was demonstrated for serum, EDTA plasma and heparin plasma. Kit components should be stored at +4°C or –20°C as instructed and the product should not be used past its expiration date.