Human ATP Binding Cassette Transporter C9 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the quantitative in vitro determination of human ATP Binding Cassette Transporter C9 in tissue homogenates, cell lysates, or other biological fluids, with reactivity confirmed for human samples. The assay employs the sandwich enzyme immunoassay technique, using an antibody specific for ATP Binding Cassette Transporter C9 that is pre-coated onto a 96-well microplate arranged as 12 x 8 well strips.

Standards and test samples are added to the wells, followed by Biotinylated Anti-ATP Binding Cassette Transporter C9 Antibody, HRP-Avidin conjugate, and TMB substrate solution. After addition of the acidic stop solution, a yellow-coloured product is produced whose absorbance at 450 nm, read on a colorimetric microplate reader, is proportional to the concentration of ATP Binding Cassette Transporter C9 captured in each well. The kit reports a product range of 0.312–20 ng/ml and a sensitivity of <0.132 ng/ml, with a total assay time of approximately 4 hours 30 minutes. Reported recovery averages for serum, EDTA plasma, and heparin plasma (n=5) are 91%, 90%, and 84% respectively, with dilution linearity data provided across 1:2 to 1:16 dilutions for each sample type. Kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date.