Human ATP Binding Cassette Transporter A4 ELISA Kit

A sandwich ELISA kit from Antibodies.com is intended for the in vitro quantitative determination of human ATP Binding Cassette Transporter A4 (ABCA4) in tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, and the procedure has a total run time of 4 hours 30 minutes. Signal is read on a colorimetric microplate reader at 450 nm, with ABCA4 concentration calculated against the supplied standard curve.

According to the manufacturer's validation data, the kit has a sensitivity of <0.112 ng/ml and a working range of 0.312–20 ng/ml. Mean recovery values were reported as 91% in serum, 90% in EDTA plasma, and 84% in heparin plasma, with linearity demonstrated across dilutions from 1:2 to 1:16 in the same matrices. Kit components should be stored at +4°C or -20°C as indicated on the individual labels and the kit should not be used past its stated expiration date.