Human Asialoglycoprotein Receptor 2 ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Asialoglycoprotein Receptor 2 (ASGR2) in serum, plasma, tissue homogenates, and other biological fluids. The assay uses a pre-coated microplate, a biotinylated anti-ASGR2 detection antibody, and an HRP-avidin conjugate, with signal developed using TMB substrate and read at 450 nm on a colorimetric microplate reader.

The standard curve covers 0.156–10 ng/ml, with a reported sensitivity of 0.049 ng/ml, and the full protocol takes approximately 4 hours 30 minutes. Recovery was validated in serum (range 80–102%, average 91%), EDTA plasma (range 81–100%, average 90%), and heparin plasma (range 80–89%, average 84%), each assessed across n=5 samples. Dilution linearity has been confirmed at 1:2, 1:4, 1:8, and 1:16 dilutions in all three matrices. Kit components are stored at +4°C or -20°C as indicated, and the kit should not be used past its expiration date.