Human Asc Type Amino Acid Transporter 1 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of human SLC7A10 (Asc-type amino acid transporter 1) in tissue homogenates, cell lysates or other biological fluids. The kit uses a pre-coated 96-well microplate (12 x 8 well strips) and employs a biotinylated anti-SLC7A10 detection antibody followed by an HRP-avidin conjugate. TMB substrate produces a blue product that turns yellow upon addition of stop solution, with absorbance read at 450 nm in a microplate reader and concentration calculated against a standard curve.

The reported assay range is 0.156–10 ng/ml with a sensitivity of 0.063 ng/ml, and the total assay time is 4 hours 30 minutes. The protocol booklet describes sample collection and storage guidance for serum, EDTA or heparin plasma, cell culture supernatant, tissue homogenates, and cell lysates, including centrifugation conditions and the recommendation that total protein concentration per well should not exceed 0.3 mg. Kit components are stored at +4 °C or -20 °C as indicated, and the product is intended for research use only.