Human Arylformamidase ELISA Kit

From Antibodies.com, this sandwich ELISA enables the in vitro quantitative determination of human Arylformamidase in tissue homogenates, cell lysates or other biological fluids. The kit is built around a pre-coated microplate arranged as 12 x 8 well strips. Captured Arylformamidase is detected using a Biotinylated Anti-Arylformamidase Antibody, an HRP-Avidin conjugate and a TMB substrate; the reaction is read at 450 nm on a colorimetric microplate reader.

Key performance figures stated by the manufacturer include a product range of 1.56–100 ng/ml, a sensitivity of 0.57 ng/ml and a total assay time of 4h 30m. Spike-and-recovery data are reported for serum (n=5, range 80–102%, average 91%), EDTA plasma (n=5, range 81–100%, average 90%) and heparin plasma (n=5, range 80–89%, average 84%), alongside dilution-linearity testing at 1:2, 1:4, 1:8 and 1:16 for the same matrices. Components are stored at +4°C or -20°C as instructed, and the kit is described as suitable for use with cell lysates and tissue homogenates; components should not be used past the expiration date.