Human Aromatase ELISA Kit

From Antibodies.com, this sandwich ELISA kit is intended for the in vitro quantitative determination of human Aromatase in serum, plasma, tissue homogenates or other biological fluids. The assay employs a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a colorimetric readout based on TMB substrate and a stop solution, measured at 450 nm in a microplate reader.

It is reactive with human samples. The stated assay range is 0.156–10 ng/ml with a sensitivity of 0.065 ng/ml. Total assay time is reported as 4 hours 30 minutes, and kit components are stored at +4°C or –20°C as instructed; the kit should not be used past its expiration date.

Spike-and-recovery performance has been reported in serum, EDTA plasma and heparin plasma (n=5 each), with recovery ranges of 80–102% (average 91%), 81–100% (average 90%) and 80–89% (average 84%) respectively, across a 1:2 to 1:16 dilution series.