Human Apolipoprotein A2 ELISA Kit

A sandwich ELISA from Antibodies.com for the in vitro quantitative determination of human Apolipoprotein A II (ApoA-II) in serum, plasma or other biological fluids. The assay uses a pre-coated microplate, a biotinylated anti-ApoA-II detection antibody and HRP-avidin, with a TMB substrate read at 450 nm on a colorimetric microplate reader. Sensitivity is <25.4 ng/ml, the assay range is 62.5–4000 ng/ml, and total assay time is 4 h 30 min. Kit components are stored at +4 °C or −20 °C as instructed.

Manufacturer-reported spike-and-recovery values: serum (n=5) average 91% across 80–102%; EDTA plasma (n=5) average 90% across 81–100%; heparin plasma (n=5) average 84% across 80–89%. Manufacturer-reported dilution linearity at 1:2, 1:4, 1:8 and 1:16 covers 74–107% for serum, 79–108% for EDTA plasma and 82–105% for heparin plasma (n=5).