Human Angiopoietin Like Protein 4 ELISA Kit

A sandwich ELISA from Antibodies.com for the quantitative in vitro determination of human Angiopoietin-like 4 (ANGPTL4). The kit is suitable for serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids, and the result is read as O.D. absorbance at 450 nm using a colorimetric microplate reader.

The assay uses a pre-coated 96-well microtiter plate, a biotinylated anti-ANGPTL4 detection antibody, an HRP-avidin conjugate and a TMB substrate. Product range is 0.781-50 ng/ml with sensitivity of <0.31 ng/ml, and total assay time is 4h 30m. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.

Validation data in the product literature report average recovery of 91% for serum (range 80%-102%, n=5), 91% for EDTA plasma (range 81%-101%, n=5) and 84% for heparin plasma (range 80%-89%, n=5), with dilutional linearity tested at 1:2, 1:4, 1:8 and 1:16 dilutions for each matrix.