Human Angiopoietin 4 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is designed for the in vitro quantitative determination of human Angiopoietin 4 in serum, plasma, tissue homogenates, or other biological fluids [S1]. The assay uses a pre-coated 96-well microplate to which standards and samples are added; the Angiopoietin 4 present in each sample is captured by the immobilized antibody, then detected with a Biotinylated Anti-Angiopoietin 4 Antibody, HRP-Avidin conjugate, and TMB substrate, producing a yellow chromogen whose absorbance is read at 450 nm in a microplate reader and referenced to a standard curve [S1].

Targeted reactivity is human and the product is classified within the Assays and Kits category [C0, S1]. The platform is a colourmetric, pre-coated microplate read on a standard microplate reader [S1]. The reported assay range is 18.7-1200 pg/ml and the sensitivity is 7.36 pg/ml [S1]. Total assay time is 4h 30m [S1]. Kit components are intended for storage at +4°C or -20°C as instructed and must not be used past the indicated expiration date [S1].

Sample-type recovery data reported by the manufacturer show average recoveries of 91% for serum, 91% for EDTA plasma, and 84% for heparin plasma [S1]. Dilution linearity ranges across 1:2, 1:4, 1:8, and 1:16 dilutions are also provided for serum (87-91%, 87-107%, 74-101%, 92-97%), EDTA plasma (90-105%, 84-101%, 90-101%, 79-108%), and heparin plasma (84-95%, 92-105%, 82-105%, 89-91%) [S1].