Human Angiogenic Factor With G Patch And FHA Domains 1 ELISA Kit

Antibodies.com supplies this sandwich ELISA (sELISA) kit for the in vitro quantitative determination of human Angiogenic Factor With G Patch And FHA Domains 1 (AGGF1) in serum, plasma, tissue homogenates, and other biological fluids. The assay is configured for use with a colorimetric microplate reader and a pre-coated 96-well microplate arranged as 12 x 8 well strips.

The workflow uses a biotinylated anti-AGGF1 detection antibody in combination with an HRP-Avidin conjugate and TMB substrate, with absorbance read at 450 nm after addition of the acidic stop solution. The manufacturer's quantitative range is 0.156–10 ng/ml and sensitivity is 0.051 ng/ml, with a total assay time of 4h 30m. Standards and samples are added to the pre-coated plate, captured by the immobilized antibody, washed, and developed in sequence.

Validation data from the manufacturer reports the following recoveries: serum (n=5) 80%–102%, average 91%; EDTA plasma (n=5) 81%–100%, average 90%; heparin plasma (n=5) 80%–89%, average 84%. Dilution linearity was assessed at 1:2, 1:4, 1:8 and 1:16 across serum, EDTA plasma and heparin plasma samples. Kit components are stored at +4°C or -20°C as instructed and should not be used past the expiration date.