Human Aminoadipate Semialdehyde Phosphopantetheinyl Transferase ELISA Kit

Manufactured by Antibodies.com, this sandwich ELISA kit is intended for the in vitro quantitative determination of human Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT) in tissue homogenates or other biological fluids. The assay uses a pre-coated microplate platform and is read on a colorimetric microplate reader at 450 nm, with sample concentrations calculated from a standard curve.

Quoted performance specifications include a sensitivity of <0.054 ng/ml and an assay range of 0.156–10 ng/ml. Spike-and-recovery data report averages of 91% in serum (80%–102%, n=5), 91% in EDTA plasma (81%–101%, n=5) and 84% in heparin plasma (80%–89%, n=5). Dilution linearity is reported across 1:2, 1:4, 1:8 and 1:16 dilutions for serum, EDTA plasma and heparin plasma samples. Total assay time is 4 h 30 m.

Kit components should be stored at +4°C or −20°C as directed, and the kit should not be used past its expiration date.