Human Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 ELISA Kit

Antibodies.com provides a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) intended for the in vitro quantitative determination of human EMAP II/AIMP1 in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips. Standards and samples are captured by an immobilized antibody and detected using a biotinylated anti-EMAP II/AIMP1 antibody followed by an HRP-Avidin conjugate. The enzymatic reaction is visualised with TMB substrate, producing a blue product that turns yellow upon addition of stop solution, and is read at 450 nm on a colorimetric microplate reader.

Performance specifications include a measurement range of 125-8000 pg/ml and a sensitivity of <43 pg/ml, with a total assay time of approximately 4 hours 30 minutes. The kit is suitable for cell lysates, tissue homogenates and cell culture supernates. Reported recovery averages from the manufacturer are 91% for serum (range 80-102%), 90% for EDTA plasma (range 81-100%) and 84% for heparin plasma (range 80-89%), each based on n=5.

Kit components include a pre-coated microplate, lyophilized standard, two detection solutions, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers. Components are stored at +4°C or -20°C as instructed and should not be used past the stated expiration date.