Human Alpha-Methylacyl Coenzyme A Racemase ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of human Alpha-Methylacyl Coenzyme A Racemase (AMACR) in tissue homogenates, cell lysates, and other biological fluids. The assay is performed on a pre-coated 96-well microplate, with colourimetric detection read at 450 nm in a microplate reader, and is reactive with human samples.

Quoted performance figures include a sensitivity of <0.059 ng/ml and an assay range of 0.156–10 ng/ml, with a total assay time of 4 hours 30 minutes. Sample-type recovery data are reported for serum, EDTA plasma, and heparin plasma. The workflow uses a Biotinylated Anti-AMACR Detection Antibody followed by HRP-Avidin conjugate, TMB substrate, and an acidic stop solution; the resulting yellow colour intensity is proportional to AMACR concentration in the well.

Kit components comprise a pre-coated 96-well microplate (12 x 8 well strips), lyophilised standard, Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution, and plate sealers, to be stored at +4°C or −20°C as instructed. The kit should not be used past the stated expiration date.