Human Alpha-2-Heremans Schmid Glycoprotein ELISA Kit

This Antibodies.com sandwich enzyme-linked immunosorbent assay (sELISA) kit is designed for the in vitro quantitative determination of human Alpha-2-Heremans Schmid Glycoprotein (AHSG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The assay employs an antibody specific for AHSG pre-coated onto a microtiter plate. Standards and test samples are added to the wells, where AHSG is captured by the immobilized antibody. Following washing, a biotinylated anti-AHSG antibody binds the captured analyte, and HRP-Avidin conjugate is then added. After further incubation and washing, TMB substrate is used to visualise the enzymatic reaction, producing a blue-coloured product that changes to yellow upon addition of acidic stop solution. Absorbance is read at 450 nm in a microplate reader and the concentration of AHSG is calculated by reference to a standard curve.

The standard range is 0.468–30 ng/ml with a sensitivity of <0.22 ng/ml. Total assay time is 4 h 30 min and detection is colorimetric. Kit components should be stored at +4 °C or −20 °C as instructed, and the kit should not be used past its expiration date. Reported mean recovery values for n=5 samples were 91% for serum (range 80%–102%), 90% for EDTA plasma (range 81%–100%) and 84% for heparin plasma (range 80%–89%). Dilution linearity has been evaluated at 1:2, 1:4, 1:8 and 1:16 dilutions across serum, EDTA plasma and heparin plasma samples.

The kit has been cited in one publication in which AHSG (fetuin-A) was investigated as a molecular signature of metabolic disorders, with associations reported for obesity, type 1 and type 2 diabetes, and liver steatosis.