Human Alpha-1-B-Glycoprotein ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human Alpha-1-B-Glycoprotein (A1BG) in serum, plasma, or other biological fluids. The assay is run on a pre-coated 96-well microplate arranged as 12 x 8 well strips and read colorimetrically at 450 nm.

The reported assay range is 0.625-40 ng/ml with a sensitivity of 0.253 ng/ml. Total assay time is 4h 30m, and kit components are stored at +4°C or -20°C as instructed.

Spike-and-recovery data (n=5) report averages of 91% for serum (range 80-102%), 90% for EDTA plasma (range 81-100%), and 84% for heparin plasma (range 80-89%). Dilution linearity at 1:2, 1:4, 1:8 and 1:16 dilutions is reported as 87-91% / 87-107% / 74-101% / 92-97% for serum, 90-105% / 84-101% / 90-101% / 79-108% for EDTA plasma, and 84-95% / 92-105% / 82-105% / 89-91% for heparin plasma.