Human Aldolase B, Fructose Bisphosphate ELISA Kit

From Antibodies.com, this human Aldolase B (Fructose Bisphosphate) ELISA kit is designed for the in vitro quantitative determination of human Aldolase B in serum, plasma, tissue homogenates and other biological fluids. The assay is configured as a sandwich ELISA with reactivity specific to human samples, and falls within the Assays and Kits product category.

The kit uses a pre-coated microplate platform with colourmetric detection read at 450 nm on a standard microplate reader. The standard curve covers an assay range of 0.156–10 ng/ml with a reported sensitivity of <0.063 ng/ml, and the total assay time is 4h 30m. Components are stored at +4°C or –20°C as indicated, and the kit must not be used past its expiration date. The procedure involves biotinylated anti-Aldolase B antibody binding, HRP-avidin conjugate addition, and TMB substrate visualisation, with the resulting yellow colour intensity proportional to the Aldolase B concentration in each well.

Recovery has been validated across three matrices: serum (n=5) averages 91% over a range of 80–102%, EDTA plasma (n=5) averages 90% over a range of 81–100%, and heparin plasma (n=5) averages 84% over a range of 80–89%. Dilution linearity has also been characterised at 1:2, 1:4, 1:8 and 1:16 dilutions across these sample types.