Human Alanine Aminopeptidase ELISA Kit

A quantitative sandwich ELISA from Antibodies.com for the in vitro determination of human Alanine Aminopeptidase (also known as CD13) in serum, plasma, tissue homogenates or other biological fluids. The assay uses a pre-coated microplate to capture the target, followed by a biotinylated detection antibody, an HRP-avidin conjugate, and a TMB substrate; the resulting yellow product is read at 450 nm on a colorimetric microplate reader.

The standard curve spans 1.56–100 ng/ml with a reported sensitivity of 0.51 ng/ml, and the full protocol is completed in approximately 4 hours 30 minutes. Kit components are stored at +4°C or –20°C as indicated, and the assay is intended for use with human samples only.