Human Adrenomedullin ELISA Kit

From Antibodies.com, this Adrenomedullin/ADM ELISA Kit is intended for the in vitro quantitative determination of human Adrenomedullin (ADM) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The procedure uses a sandwich enzyme immunoassay format on a pre-coated 96-well microplate configured as 12 x 8 well strips.

The manufacturer states a product range of 15.6-1000 pg/ml and a sensitivity of <4.77 pg/ml. Detection is colorimetric, with absorbance read at 450 nm in a microplate reader, and total assay time is 4 hours 30 minutes. Captured analyte is detected with a biotinylated anti-Adrenomedullin/ADM antibody and an HRP-avidin conjugate, and a TMB substrate produces a yellow product whose intensity is proportional to ADM concentration in each well.

Manufacturer recovery data (n=5) report ranges of 80%-102% (average 91%) for serum, 81%-100% (average 90%) for EDTA plasma and 80%-89% (average 84%) for heparin plasma, with linearity tested at dilutions of 1:2, 1:4, 1:8 and 1:16. Supplied components include a pre-coated 96-well microplate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, with storage between +4°C and -20°C as instructed.