Human Adrenergic Receptor Alpha 1A ELISA Kit

Antibodies.com supplies a sandwich ELISA for the in vitro quantitative determination of human alpha 1a Adrenergic Receptor (ADRA1A) in tissue homogenates, cell lysates and other biological fluids, with reactivity against human samples. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips together with a biotinylated anti-ADRA1A detection antibody, HRP-avidin conjugate and TMB substrate; the reaction is read at 450 nm in a colorimetric microplate reader.

The kit has a stated product range of 0.312–20 ng/ml and a sensitivity of <0.116 ng/ml, with a total assay time of 4h 30m. Reported spike-and-recovery from serum (n=5) was 83–100% with an average of 91%, from EDTA plasma (n=5) was 90–98% with an average of 94%, and from heparin plasma (n=5) was 89–102% with an average of 95%. Kit components are to be stored at +4°C or -20°C as indicated and not used past the expiration date.

Supplied items include a pre-coated 96 well microplate, lyophilized standard, detection solution A, detection solution B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers.