Human Activin Receptor Like Kinase 1 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of human Activin Receptor Like Kinase 1 (ALK-1) in tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated 96-well microplate and employs a colourmetric detection method with absorbance read at 450 nm.

Manufacturer specifications report a sensitivity of 0.054 ng/ml and an assay range of 0.156-10 ng/ml, with a total assay time of 4h 30m. The kit is supplied with a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers, with storage temperatures varying between +4°C and -20°C depending on the component.

Spike-and-recovery performance was reported in serum (80%-102%, average 91%), EDTA plasma (81%-100%, average 90%), and heparin plasma (80%-89%, average 84%) across five samples per matrix. Dilution linearity across 1:2, 1:4, 1:8, and 1:16 ratios yielded overall recovery ranges of 74-107% for serum, 79-108% for EDTA plasma, and 82-105% for heparin plasma.