Human Absent In Melanoma 1 ELISA Kit

A sandwich enzyme-linked immunosorbent assay (sELISA) designed for the in vitro quantitative determination of human AIM1 (Absent In Melanoma 1) in tissue homogenates, cell lysates and other biological fluids, as well as serum, plasma and cell culture supernatant samples. Antibodies.com supplies the kit as a quantitative sandwich immunoassay built around a pre-coated 96-well microplate arranged as 12 x 8 well strips, with absorbance read at 450 nm on a standard colorimetric microplate reader. The stated assay range is 0.312–20 ng/ml and the sensitivity is 0.134 ng/ml. Total assay time is 4 hours 30 minutes, with kit components stored at +4°C or -20°C as instructed. Components include lyophilized standard, biotinylated detection antibodies, HRP-avidin conjugate, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution and plate sealers.

Spike-and-recovery data provided with the kit report average recoveries of 91% in serum (range 80–102%, n=5), 90% in EDTA plasma (range 81–100%, n=5) and 84% in heparin plasma (range 80–89%, n=5). Dilutional linearity was assessed at 1:2, 1:4, 1:8 and 1:16 across the same sample types. The product is intended for research use only and is not for diagnostic or therapeutic applications.