Human A Disintegrin And Metalloproteinase With Thrombospondin 9 ELISA Kit

This sandwich ELISA kit from Antibodies.com is intended for the in vitro quantitative determination of human ADAMTS9 in tissue homogenates, cell lysates and other biological fluids. The assay is run on a pre-coated 96-well microplate configured as 12 × 8-well strips and uses a biotinylated anti-ADAMTS9 detection antibody with HRP-avidin and TMB substrate; absorbance is read at 450 nm in a microplate reader.

The reported assay range is 0.312–20 ng/ml with a sensitivity of <0.109 ng/ml, and the full protocol takes approximately 4 hours 30 minutes.

Recovery was assessed in serum (80–102%, average 91%, n=5), EDTA plasma (81–101%, average 91%, n=5) and heparin plasma (80–89%, average 84%, n=5). Linearity on serial dilution (1:2 to 1:16) was evaluated across the same sample types. Kit components are stored at +4°C or −20°C as instructed, and the kit should not be used past its expiration date.