Human A Disintegrin And Metalloproteinase With Thrombospondin 4 ELISA Kit

Antibodies.com offers this Human ADAMTS4 Sandwich ELISA Kit for the in vitro quantitative determination of human ADAMTS4 in serum, plasma, tissue homogenates or other biological fluids. The assay employs the sandwich enzyme immunoassay technique, with an antibody specific for ADAMTS4 pre-coated onto a 96-well microplate arranged as 12 x 8 well strips. Standards and test samples are added to the wells; bound ADAMTS4 is then detected using a Biotinylated Anti-ADAMTS4 Antibody followed by HRP-Avidin conjugate. The enzymatic reaction is visualised with TMB substrate, producing a blue-coloured product that turns yellow upon addition of acidic stop solution, with absorbance read at 450 nm in a microplate reader and the concentration calculated against the standard curve.

Manufacturer-reported performance characteristics include a sensitivity of 26.3 pg/ml and an assay range of 62.5-4000 pg/ml, with a total assay time of 4h 30m. Spike-and-recovery was assessed in serum (n=5) at 80%-102% averaging 91%, in EDTA plasma (n=5) at 81%-101% averaging 91%, and in heparin plasma (n=5) at 80%-89% averaging 84%. Dilution linearity was demonstrated at 1:2, 1:4, 1:8 and 1:16 across serum, EDTA plasma and heparin plasma samples. Detection is colourimetric on a microplate reader, and kit components are stored at +4°C or -20°C as instructed; the kit should not be used past its expiration date.