Human A Disintegrin And Metalloproteinase With Thrombospondin 1 ELISA Kit

This sandwich ELISA (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of human ADAMTS1 (A Disintegrin And Metalloproteinase With Thrombospondin 1) in tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated 96-well microplate and employs a biotinylated anti-ADAMTS1 detection antibody, an HRP-Avidin conjugate, and TMB substrate. The HRP-catalysed reaction produces a blue product that changes to yellow after addition of acidic stop solution, with absorbance read at 450 nm in a microplate reader.

According to the supplied data, the kit has an assay range of 0.781–50 ng/ml and a sensitivity of 0.27 ng/ml, with a total assay time of 4 hours 30 minutes. Spike-and-recovery in validation samples (n=5) was: serum 80–102% (average 91%), EDTA plasma 81–101% (average 91%), and heparin plasma 80–89% (average 84%). Linearity was demonstrated across 1:2, 1:4, 1:8, and 1:16 dilutions in serum, EDTA plasma, and heparin plasma. The product is intended for research use only and is not for diagnostic or therapeutic applications.