Human A Disintegrin And Metalloprotease 9 ELISA Kit

This sandwich ELISA from Antibodies.com enables the in vitro quantitative determination of human ADAM9 in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The assay uses a 96-well microplate pre-coated with an antibody specific for ADAM9. Standards and samples are added to the wells, and captured analyte is detected using a biotinylated anti-ADAM9 antibody followed by an HRP-avidin conjugate and TMB substrate, producing a colour change that is read at 450 nm on a colorimetric microplate reader.

The kit's stated measuring range is 0.156–10 ng/ml with a sensitivity of <0.064 ng/ml, and the complete protocol takes approximately 4 hours 30 minutes. Kit components are stored at +4°C or –20°C as instructed. Reported recovery in neat samples (n=5) is 80–102% (average 91%) for serum, 81–100% (average 90%) for EDTA plasma, and 80–89% (average 84%) for heparin plasma.