Human A Disintegrin And Metalloprotease 28 ELISA Kit

Offered by Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (ELISA) is designed for the in vitro quantitative determination of human A Disintegrin And Metalloprotease 28 (ADAM28) in serum, plasma, tissue homogenates or other biological fluids. The assay employs a pre-coated 96-well microplate format with a biotinylated detection antibody and HRP-based colorimetric detection, producing a yellow product whose absorbance is read at 450 nm in a microplate reader and referred to a standard curve.

Reported performance characteristics include a sensitivity of 0.061 ng/ml and an assay range of 0.156–10 ng/ml. The procedure uses TMB substrate with an acidic stop solution, and the complete assay can be completed in approximately 4 hours 30 minutes. Kit components are stored at +4°C or −20°C as instructed in the protocol, and the product is shipped at +4°C.

Validated sample matrices include serum, EDTA plasma and heparin plasma, for which recovery data are provided across a range of dilution factors. The product is intended for research use only and is not for diagnostic or therapeutic applications.