Human A Disintegrin And Metalloprotease 22 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of human ADAM22 (A Disintegrin And Metalloprotease 22) in serum, plasma, tissue homogenates or other biological fluids. The assay employs a 96-well microplate pre-coated with an antibody specific for ADAM22, and uses a biotinylated detection antibody followed by an HRP-avidin conjugate with TMB substrate for colourmetric readout at 450 nm.

Sensitivity is reported as <0.057 ng/ml, with a product range of 0.156-10 ng/ml. Total assay time is 4 hours 30 minutes, and the detection type is colourmetric. Kit components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.

Recovery data are provided: in serum (n=5) the average range is 83%-100% with 91% mean; in EDTA plasma (n=5) 90%-98% with 94% mean; in heparin plasma (n=5) 90%-99% with 94% mean. Dilution linearity results (1:2, 1:4, 1:8, 1:16) are also reported for serum (90-99%, 82-108%, 82-96%, 92-103%), EDTA plasma (84-105%, 88-94%, 89-107%, 72-106%) and heparin plasma (89-92%, 88-108%, 84-102%, 90-98%).