Human A Disintegrin And Metalloprotease 19 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of human ADAM19 (A Disintegrin And Metalloprotease 19) in tissue homogenates or other biological fluids. The assay employs a pre-coated microplate format and uses a biotinylated anti-ADAM19 detection antibody with an HRP-avidin conjugate, with signal developed using TMB substrate solution and read at 450 nm on a colorimetric microplate reader.

The assay has a quantitative range of 0.312–20 ng/ml with a sensitivity of <0.108 ng/ml and a total run time of 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.

Spike-and-recovery performance has been validated across serum (average 91%, range 83–100%), EDTA plasma (average 94%, range 90–98%), and heparin plasma (average 95%, range 89–102%), with dilution linearity demonstrated at 1:2, 1:4, 1:8, and 1:16 in the same sample types.