Human 5-Hydroxytryptamine Receptor 4 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of human 5HT4 Receptor (5-Hydroxytryptamine Receptor 4) in tissue homogenates, cell lysates and other biological fluids. The assay uses the sandwich enzyme immunoassay technique on a pre-coated 96-well microplate arranged as 12 x 8 well strips, with colorimetric detection read at 450 nm in a microplate reader.

Reported sensitivity is 0.063 ng/ml and the standard curve range is 0.156-10 ng/ml, with a total assay time of 4 hours 30 minutes. After incubation with biotinylated anti-5HT4 receptor antibody and an HRP-avidin conjugate, TMB substrate is added; the resulting yellow product intensity is proportional to 5HT4 receptor concentration in each well. Reported recovery ranges are 80%-102% for serum (n=5, average 91%), 81%-100% for EDTA plasma (n=5, average 90%) and 80%-89% for heparin plasma (n=5, average 84%).

Supplied components include the pre-coated microplate, lyophilized standard (2 vials), Detection Solutions A and B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and adhesive plate sealers, stored at +4°C or -20°C as instructed. The product is for research use only and is not intended for diagnostic or therapeutic applications.