Human 11-Beta-Hydroxysteroid Dehydrogenase Type 2 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com, intended for the in vitro quantitative determination of human 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11B2) in tissue homogenates, cell lysates, or other biological fluids.

The assay employs a pre-coated microplate (12 x 8 well strips) onto which an antibody specific for HSD11B2 has been immobilised. Standards and test samples are added to the wells, and any HSD11B2 present is captured by the immobilised antibody. After washing, a Biotinylated Anti-HSD11B2 Antibody is added, followed by an HRP-Avidin conjugate. TMB substrate is then used to visualise the HRP enzymatic reaction, producing a blue product that changes to yellow upon addition of an acidic stop solution. The density of yellow is proportional to the amount of HSD11B2 captured in each well, and the concentration is calculated by reading the O.D. absorbance at 450 nm in a microplate reader against a standard curve.

The reported assay range is 0.156–10 ng/ml with a sensitivity of 0.057 ng/ml. The complete assay time is 4 hours 30 minutes, and detection is colourimetric. Kit components should be stored at +4°C or -20°C as instructed, and the kit must not be used past its expiration date. For human samples (n=5), average recovery was 91% in serum (range 83–99%), 90% in EDTA plasma (range 80–100%), and 90% in heparin plasma (range 82–99%).