HRS (phospho Tyr334) Cell Based ELISA Kit

From Antibodies.com, this cell-based ELISA kit belongs to the Assays and Kits product category and is designed for the indirect in vitro quantitative determination of HRS (phospho Tyr334) levels in adherent and suspension cells. The kit targets the HGS protein with phospho Tyr334 modification and shows reactivity with Human, Mouse, and Rat samples at the Tyr334 site. No cross-reactivity has been reported.

The assay uses a colorimetric detection method on a tissue culture treated microplate platform, with an assay time of 4 hours 30 minutes. Qualitative determination of HRS (phospho Tyr334) concentration is achieved by an indirect ELISA format, where Anti-HRS (phospho Tyr334) antibodies capture the target, which is then detected by HRP-conjugated secondary antibodies catalysing a colorimetric reaction upon substrate addition. Normalization methods include an internal positive control with Anti-GAPDH antibody, Crystal Violet whole-cell staining to determine cell density, and Anti-HRS antibody for normalizing the absorbance values of the phosphorylated target to the non-phosphorylated target.

The kit has an assay range of > 5000 Cells and should be stored at +4°C, where it remains stable for 3-6 months. An easy-to-follow, ready-to-use protocol is provided.