Horse Irisin ELISA Kit

From Antibodies.com, this Sandwich ELISA assay is designed for the in vitro quantitative determination of horse Irisin/FNDC5 in serum, plasma, tissue homogenates, and other biological fluids. It is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) carried out on a pre-coated microplate arranged as 12 x 8 well strips, and the product falls within the Assays and Kits category.

The assay is reported as specific for Irisin/FNDC5, with no reported cross-reactivity with other analogs or variants. Detection is colorimetric, based on absorbance readings at 450 nm following a TMB substrate reaction. The product range is 15.625-1000 ng/mL with a stated sensitivity of 9.375 ng/mL.

Recovery has been validated across serum, EDTA plasma, and heparin plasma samples (n=10 per matrix), with average recovery values of 96% for serum, 98% for EDTA plasma, and 95% for heparin plasma. Linearity has likewise been assessed at 1:2, 1:4, and 1:8 dilutions across the same matrices. The kit is shipped at +4°C, with components to be stored at +4°C or -20°C as instructed in the protocol manual.