Guinea pig MYO ELISA Kit

A sandwich enzyme-linked immunosorbent assay (sELISA) supplied by Antibodies.com for the in vitro quantitative determination of guinea pig Myoglobin. The assay is validated for use with serum, plasma, tissue homogenates, and other biological fluids, and provides a detection range of 1.563–100 ng/mL with a sensitivity of 0.938 ng/mL.

The kit employs a 96-well microplate pre-coated with an antibody specific for Myoglobin. Standards and samples are added to the wells and bound Myoglobin is detected using a biotinylated anti-Myoglobin antibody followed by an HRP-Streptavidin conjugate and TMB substrate. Absorbance is read at 450 nm and the Myoglobin concentration is calculated by reference to a standard curve. The procedure includes a 90-minute incubation at 37°C following sample addition.

All kit components should be stored at +4°C and the product is intended for research use only. Reagents provided include a pre-coated 96-well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody, antibody dilution buffer, HRP-Streptavidin conjugate, SABC dilution buffer, TMB substrate, stop solution, wash buffer, and plate sealers.