Guinea Pig Gastrin ELISA Kit

Antibodies.com provides a sandwich-format ELISA for the quantitative in vitro determination of guinea pig Gastrin in serum, plasma or other biological fluids. The assay employs a pre-coated 96-well microplate arranged as 12 x 8 well strips, with colorimetric detection read at 450 nm in a microplate reader. The product range is 15.6-1000 pg/ml and sensitivity is 5.9 pg/ml; the complete protocol runs in 4h 30m.

Standards and samples are added to antibody-coated wells, followed by Biotinylated Anti-Gastrin Antibody and an HRP-Avidin conjugate. TMB substrate is added and the reaction is stopped with acidic stop solution; the resulting yellow colour intensity is proportional to Gastrin concentration and is quantified against a standard curve. Reported average recovery is 91% for serum (range 80-102%, n=5), 90% for EDTA plasma (range 81-99%, n=5) and 84% for heparin plasma (range 80-89%, n=5), with dilution linearity data provided across 1:2 to 1:16 dilutions for each sample type.

Components supplied include a pre-coated 96 well microplate (12 x 8 well strips), lyophilized standard (2 vials), detection solution A (120 μl), detection solution B (120 μl), 30X wash buffer (20 ml), sample dilution buffer (45 ml), TMB substrate (9 ml), stop solution (6 ml), and plate sealers (5 adhesive strips). Storage is at +4°C or -20°C as instructed, and the kit should not be used past the expiration date.