Guinea Pig Cholecystokinin 8, Octapeptide ELISA Kit

From Antibodies.com, this competitive ELISA kit is designed for the in vitro quantitative determination of guinea pig Cholecystokinin 8, Octapeptide in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. The assay employs a competitive enzyme immunoassay technique on a pre-coated 96-well microplate supplied in a 12 x 8 well strip format, with absorbance read at 450 nm on a colorimetric microplate reader.

The kit has a stated product range of 15.6–1000 pg/ml and a sensitivity of less than 5.28 pg/ml, with a total assay time of 2 h 30 min. Kit components should be stored at +4 °C or −20 °C as instructed, and the kit should not be used past the expiration date.

Validation data supplied by the manufacturer includes spike-and-recovery results for serum (n=5, range 80–102%, average 91%), EDTA plasma (n=5, range 81–100%, average 90%) and heparin plasma (n=5, range 80–89%, average 84%), together with dilution linearity at 1:2, 1:4, 1:8 and 1:16 across the same sample types.