General Vitamin D2 ELISA Kit

This competitive Enzyme-Linked Immunosorbent Assay (cELISA) from Antibodies.com is designed for the in vitro quantitative determination of Vitamin D2 in serum, plasma, tissue homogenates and other biological fluids. The 96-well microplate is pre-coated with Vitamin D2 antigen, and sample analyte competes with the immobilised antigen for binding sites on a biotinylated anti-Vitamin D2 antibody. Detection uses an HRP-avidin conjugate and TMB substrate, producing a yellow colour whose intensity is read at 450 nm and is inversely proportional to Vitamin D2 concentration.

The assay reports a sensitivity of less than 2.2 ng/ml and a quantitative range of 4.68–300 ng/ml, with a total run time of 2 hours 30 minutes on a pre-coated microplate arranged as 12 x 8 well strips. Components include lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers, stored at +4°C or −20°C as indicated.

Performance validation in serum (n=5) returned an average of 91% (range 80–102%), in EDTA plasma (n=5) 90% (81–100%), and in heparin plasma (n=5) 84% (80–89%). Dilution linearity was evaluated at 1:2, 1:4, 1:8 and 1:16 across the three sample types, supporting the kit's use with serum, plasma and tissue homogenates.