General Thromboxane A2 ELISA Kit

This competitive ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of Thromboxane A2 in serum, plasma, or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and follows a competitive enzyme immunoassay format in which sample Thromboxane A2 competes with immobilized antigen for binding sites on a biotinylated anti-Thromboxane A2 antibody. Detection is achieved with an HRP-Avidin conjugate and TMB substrate, producing a colourmetric signal that is read at 450 nm and is inversely proportional to the analyte concentration.

The reported assay range is 15.6–1000 pg/ml, with a sensitivity of <5.62 pg/ml and a total assay time of approximately 2 h 30 min. The kit is suitable for serum and plasma samples, with components stored at +4 °C or -20 °C as instructed.