General Salmon Calcitonin ELISA Kit

This competitive Enzyme-Linked Immunosorbent Assay (cELISA) from Antibodies.com is designed for the in vitro quantitative determination of Calcitonin in biological fluids. The kit uses salmon calcitonin as the target antigen and shows broad reactivity, making it suitable for detecting calcitonin across species in serum, EDTA plasma, and heparin plasma samples.

The assay employs the competitive enzyme immunoassay technique on a pre-coated microplate. During incubation, calcitonin in samples or standards competes with immobilised antigen for binding sites on a biotinylated anti-calcitonin antibody. Detection uses an HRP-avidin conjugate with TMB substrate, and absorbance is read at 450 nm. The assay range is 0.312–20 ng/ml with a sensitivity of <0.085 ng/ml. Total assay time is 2 hours 30 minutes.

Spike-and-recovery values in five replicates ranged from 80–102% (average 91%) for serum, 81–100% (average 90%) for EDTA plasma, and 80–89% (average 84%) for heparin plasma. Dilution linearity across 1:2 to 1:16 was also demonstrated for each sample type.